Home » 今村拓也 (Takuya Imamura)

今村拓也 (Takuya Imamura) 

略歴

1997年 東京大学農学部獣医学科卒業(指導教官:小川智也)

2001年 東京大学大学院農学生命科学研究科獣医学専攻博士課程修了(指導教官:塩田邦郎)

2001年 日本学術振興会特別研究員(PD:東京大学)

2002年 ジャックモノー研究所ポストドクトラルフェロー(メンター:Andras PALDI)

2003年 日本学術振興会海外特別研究員(ジャックモノー研究所)

2004年 基礎生物学研究所・助手(メンター:森裕司)

2007年 基礎生物学研究所・助教

2008年 京都大学理学研究科生物科学専攻グローバルCOEプログラム・准教授(メンター:阿形清和)

2012年 京都大学理学研究科生物科学専攻・研究員

2013年 九州大学医学研究院・助教(メンター:中島欽一)

2015年 九州大学医学研究院・准教授(統合的組織修復医学分野)

 

研究業績

(英文抜粋, *corresponding author, #equal contribution)

DNA methylome analysis identifies transcription factor-based epigenomic signatures of multilineage competence in neural stem/progenitor cells. Tsukasa Sanosaka#, Takuya Imamura#*, Nobuhiko Hamazaki, MuhChyi Chai, Katsuhide Igarashi, Maky Ideta-Otsuka, Fumihito Miura, Takashi Ito, Nobuyuki Fujii, Kazuho Ikeo, Kinichi Nakashima. Cell Reports, 20:2992 (2017) http://www.cell.com/cell-reports/fulltext/S2211-1247(17)31228-7 (open access)

Epigenetic regulation of neural stem cell differentiation towards spinal cord regeneration. Tomonori Kameda, Takuya Imamura, Kinichi Nakashima. Cell and Tissue Research, Epub ahead of print

Evolutionary acquisition of promoter-associated non-coding RNA (pancRNA) repertoires diversifies species-dependent gene activation mechanisms in mammals. Masahiro Uesaka, Kiyokazu Agata, Takao Oishi, Kinichi Nakashima, Takuya Imamura*. BMC Genomics, 18:285 (2017) https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3662-1 (open access)

Detection of bidirectional promoter-derived lncRNAs from small-scale samples using pre-amplification-free directional RNA-seq method. Nobuhiko Hamazaki, Kinichi Nakashima, Katsuhiko Hayashi, Takuya Imamura*. Methods in Molecular Biology, 1605: 83 (2017)

Highlighted in: Directional RNA-seq libraries from small samples without pre-amplification for the quantification of lncRNAs. RNA-seq blog (2017) http://www.rna-seqblog.com/rna-seq-libraries-from-small-samples-without-pre-amplification-for-the-quantification-of-lncrnas

Manipulation of promoter-associated noncoding RNAs in mouse early embryos for controlling sequence-specific epigenetic status. Nobuhiko Hamazaki, Kinichi Nakashima, Takuya Imamura*. Methods in Molecular Biology, 1543: 271 (2017)

Bidirectional promoters link cAMP signaling with irreversible differentiation through promoter-associated non-coding RNA (pancRNA) expression in PC12 cells. Naoki Yamamoto, Kiyokazu Agata, Kinichi Nakashima, Takuya Imamura*. Nucleic Acids Research, 44: 5105 (2016) http://nar.oxfordjournals.org/content/44/11/5105 (open access)

Reconstitution in vitro of the entire cycle of the mouse female germ line. Orie Hikabe, Nobuhiko Hamazaki, Go Nagamatsu, Yayoi Obata, Yuji Hirao, Norio Hamada, So Shimamoto, Takuya Imamura, Kinichi Nakashima, Mitinori Saitou, Katsuhiko Hayashi. Nature, 539: 299 (2016)

Gene activation-associated long noncoding RNAs function in mouse preimplantation development. Nobuhiko Hamazaki, Masahiro Uesaka, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura*. Development, 142: 910 (2015) http://dev.biologists.org/content/142/5/910.abstract (open access)

Highlighted in: pancRNAs in early development. Development, 142: e0501 (2015) http://dev.biologists.org/content/142/5/e0501.full

Highlighted in: Long Non-Coding RNAs take aim at methyl marks in early embryo. Biol Reprod, 92 (5): 112, 1-1 (2015) https://doi.org/10.1095/biolreprod.115.129080

Identification of hypothalamic arcuate nucleus-specific enhancer region of kiss1 gene in mice. Teppei Goto, Junko Tomikawa, Kana Ikegami, Shiori Minabe, Hitomi Abe, Tatsuya Fukanuma, Takuya Imamura, Kenji Takase, Makoto Sanbo, Koichi Tomita, Masumi Hirabayashi, Kei-ichiro Maeda, Hiroko Tsukamura, Yoshihisa Uenoyama. Mol Endocrinol, 29: 121 (2015)

miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes. Keita Tsujimura, Koichiro Irie, Hideyuki Nakashima, Yoshihiro Egashira, Yoichiro Fukao, Masayuki Fujiwara, Masayuki Itoh, Masahiro Uesaka, Takuya Imamura, Yasukazu Nakahata, Yui Yamashita, Takaya Abe, Shigeo Takamori, Kinichi Nakashima. Cell Rep, 12: 1887 (2015)

Epigenetic setting and reprogramming for neural cell fate determination and differentiation. Takuya Imamura, Masahiro Uesaka, Kinichi Nakashima. Philos Trans R Soc Lond B Biol Sci, 369: 1652 (2014)

Bidirectional promoters are the major source of gene activation-associated non-coding RNAs in mammals. Masahiro Uesaka, Osamu Nishimura, Yasuhiro Go, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura*. BMC Genomics, 15: 35 (2014) http://www.biomedcentral.com/1471-2164/15/35 (open access)

Epigenetic regulation of Kiss1 gene expression mediating estrogen-positive feedback action in the mouse brain. Junko Tomikawa, Yoshihisa Uenoyama, Makiko Ozawa, Tatsuya Fukanuma, Kenji Takase, Teppei Goto, Hitomi Abe, Nahoko Ieda, Shiori Minabe, Chikaya Deura, Naoko Inoue, Makoto Sanbo, Koichi Tomita, Masumi Hirabayashi, Satoshi Tanaka, Takuya Imamura, Hiroaki Okamura, Kei-ichiro Maeda, Hiroko Tsukamura. Proc Natl Acad Sci USA, 109: E1294 (2012)

Single-stranded noncoding RNAs mediate local epigenetic alterations at gene promoters in rat cell lines. Junko Tomikawa, Hiroko Shimokawa, Masahiro Uesaka, Naoki Yamamoto, Yuji Mori, Hiroko Tsukamura, Kei-ichiro Maeda, Takuya Imamura*. J Biol Chem 286: 34788 (2011) http://www.jbc.org/content/286/40/34788.full (open access)

Epigenetic setting for long-term expression of estrogen receptor α and androgen receptor in cells. Takuya Imamura*. Hormones and Behavior 59: 345 (2011)

Cell- to species-level diversity of epigenetic setting for androgen receptor expression in mammals. Masahiro Uesaka, Takuya Imamura*. Journal of Steroids and Hormonal Science S2: 004 (2011)

Involvement of brain ketone bodies and the noradrenergic pathway in diabetic hyperphagia in rats. Kinuyo Iwata, Mika Kinoshita, Shunji Yamada, Takuya Imamura, Yoshihisa Uenoyama, Hiroko Tsukamura, Kei-ichiro Maeda. J Physiol Sci 61: 103 (2011)

Epigenetic processes in a tetraploid mammal. Caroline Bacquet, Takuya Imamura, Claudio A. Gonzalez, Ivan Conejeros, Gudrun Kausel, Thi My Anh Neildez-Nguyen, Andras Paldi, Milton H. Gallardo. Mammalian Genome 19: 439 (2008)

Genomic Imprinting. Takuya Imamura, Andras Paldi. In: D. Ganten, K. Ruckpaul (eds), ISBN 978-3540309543 Encyclopedic Reference of Genomics and Proteomics, Springer, Heidelberg, (2006)

Dynamic CpG and non-CpG methylation of the Peg1/Mest gene in the mouse oocyte and preimplantation embryo. Takuya Imamura, Antoine Kerjean, Thomas Heams, Jean-Jacques Kupiec, Catherine Thenevin, Andras Paldi. J Biol Chem 280: 20171 (2005) http://www.jbc.org/content/280/20/20171.full (open access)

Identification of genetic and epigenetic similarities of SPHK1/Sphk1 in mammals. Takuya Imamura, Nanami Miyauchi-Senda, Satoshi Tanaka, Kunio Shiota. J Vet Med Sci 66: 1387 (2004) https://www.jstage.jst.go.jp/article/jvms/66/11/66_11_1387/_article (open access)

Non-coding RNA directed DNA demethylation of Sphk1 CpG island. Takuya Imamura, Soshi Yamamoto, Jun Ohgane, Naka Hattori, Satoshi Tanaka, Kunio Shiota. Biochem Biophys Res Comm 322: 593 (2004)

Essential Role for poly(ADP-ribosyl)ation in mouse preimplantation development. Takuya Imamura, Thy Mi Anh Neildez, Catherine Thenevin, Andras Paldi. BMC Mol Biol 5: 4 (2004) https://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-5-4 (open access)

Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice. Kunio Shiota, Yasushi Kogo, Jun Ohgane, Takuya Imamura, Atsushi Urano, Koichiro Nishino, Satoshi Tanaka, Naka Hattori. Genes Cells 7: 961 (2002)

CpG island of rat sphingosine kinase-1 gene: Tissue dependent DNA methylation status and multiple alternative first exons. Takuya Imamura, Jun Ohgane, Seiichiro Ito, Tomoya Ogawa, Naka Hattori, Satoshi Tanaka, Kunio Shiota. Genomics 76: 117 (2001)

Generation and characterization of a monoclonal antibody recognizing a fetal brain enriched O-linked sialoglycoprotein, FOG100. Takuya Imamura, Naka Hattori, Kunio Shiota, Tamoya Ogawa. J Biochem 124: 534 (1998) https://www.jstage.jst.go.jp/article/biochemistry1922/124/3/124_3_534/_article (open access)

Molecular cloning of rat leukemia inhibitory factor receptor alpha-chain and its expression during pregnancy. Jun-ichi Aikawa, Sakae Ikeda, Kwan-Sik Min, Jun Ohgane, Takuya Imamura, Ken Sasai, Kunio Shiota, Tomoya Ogawa. Biochim Biophys Acta 1353: 266 (1997)

 

(和文抜粋)

lncRNAの進化と種特異性 亀田朋典・中島欽一・今村拓也*(分担) 「ノンコーディングRNA -RNA分子の全体像を俯瞰する-」廣瀬哲郎・泊 幸秀(編集)ISBN978-4-7598-1725-6 pp.328-338, 化学同人 (2016)

R+rGADEM 今村拓也*(分担) 「細胞工学別冊 次世代シークエンサー DRY 解析教本」清水厚志・坊農秀雅(編集)ISBN978-4-7809-0920-3 pp.322-325, 秀潤社 (2015)

脳の性差から生物多様性へ 今村拓也*(分担) 「生き物たちのつづれ織り」阿形清和・森哲(監修)ISBN978-4-87698-243-1 下巻pp.90-100, 京都大学学術出版会 (2012)

Noncoding RNAによるエピジェネティック制御 今村拓也* 化学と生物 45: 211 (2007) https://www.jstage.jst.go.jp/article/kagakutoseibutsu1962/45/3/45_3_211/_pdf

Inverse PCR/ゲノムライブラリークローンの利用/データベースの利用/バイサルファイト反応とシークエンス解析によるDNAメチル化解析/5—アザシチジンとTSA処理/アンチセンスRNA発現の解析 今村拓也*(分担) 「DNAメチル化研究法」 塩田邦郎・服部中(編) ISBN 4-7622-3052-9 pp. 49-54, 55-62,62-70,85-93, 105-107,138-148, 学会出版センター (2006)

バイサルファイトシークエンス法によるDNAメチル化解析 今村拓也* 日本比較内分泌学会ニュース = Newsletter of Japan Society for Comparative Endocrinology 113: 25 (2004) https://www.jstage.jst.go.jp/article/nl2001jsce/2004/113/2004_113_113_25/_pdf

DNAメチル化による発生プログラム 今村拓也*・塩田邦郎  Molecular Medicine 39(7): 809 (2002)

 

受賞

日本獣医学会生理学・生化学分科会奨励賞 2011年9月

「マカクサルにおける偽遺伝子由来promoter-associated noncoding RNA(pancRNA)による種特異的転写活性化」

 

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